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Diverse retrotransposon families and an AT-rich satellite DNA revealed in giant genomes of Fritillaria lilies

SYSNO ASEP	0359134
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Diverse retrotransposon families and an AT-rich satellite DNA revealed in giant genomes of Fritillaria lilies
Author(s)	Ambrožová, K. (CZ) Mandáková, T. (CZ) Bureš, P. (CZ) Neumann, Pavel (BC-A)_{RID, ORCID} Leitch, I. J. (GB) Koblížková, Andrea (BC-A) Macas, Jiří (BC-A)_{RID, ORCID, SAI} Lysák, M. (CZ)
Source Title	Annals of Botany. - : Oxford University Press - ISSN 0305-7364 Roč. 107, č. 2 (2011), s. 255-268
Number of pages	14 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Fritillaria ; Liliaceae ; repetitive DNA
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA521/07/0284 GA ČR - Czech Science Foundation (CSF)
	LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50510513 - UMBR-M, BC-A (2005-2011)
UT WOS	000286672500007
DOI	10.1093/aob/mcq235
Annotation	Background and Aims The genus Fritillaria (Liliaceae) comprises species with extremely large genomes (1C = 30 000-127 000 Mb) and a bicontinental distribution. Most North American species (subgenus Liliorhiza) differ from Eurasian Fritillaria species by their distinct phylogenetic position and increased amounts of heterochromatin. This study examined the contribution of major repetitive elements to the genome obesity found in Fritillaria and identified repeats contributing to the heterochromatin arrays in Liliorhiza species. Methods Two Fritillaria species of similar genome size were selected for detailed analysis, one from each phylogeographical clade: F. affinis (1C = 45.6 pg, North America) and F. imperialis (1C = 43.0 pg, Eurasia). Fosmid libraries were constructed from their genomic DNAs and used for identification, sequence characterization, quantification and chromosome localization of clones containing highly repeated sequences.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1