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Characterization of the capillary network in skeletal muscles from 3D data
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SYSNO ASEP 0359123 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Characterization of the capillary network in skeletal muscles from 3D data Author(s) Eržen, I. (SI)
Janáček, Jiří (FGU-C) RID, ORCID
Kubínová, Lucie (FGU-C) RID, ORCIDSource Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 60, č. 1 (2011), s. 1-13Number of pages 13 s. Language eng - English Country CZ - Czech Republic Keywords capillaries ; skeletal muscle ; confocal microscopy ; stereology ; 3D analysis ; 3D visualization Subject RIV BO - Biophysics R&D Projects MEB090910 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50110509 - FGU-C (2005-2011) UT WOS 000288563800001 Annotation Immunohistochemical methods for visualization of capillaries and muscle fibres in thick muscle sections are presented. Applying confocal microscopy and virtual 3D stereological grids, or tracing of capillaries in virtual reality, length of capillaries within a muscle volume or length of capillaries adjacent to a muscle fibre per fibre length, fibre surface area or fibre volume are evaluated and 3D models of capillaries and muscle fibres are produced. The described 3D methodology is applied to the anatomical remodelling of capillarity during acute denervation and early reinnervation in the rat soleus and extensor digitorum longus muscles Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2012
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