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Preparation of stable Pd nanoparticles with tunable size for multiple immunolabeling in biomedicine

SYSNO ASEP	0359122
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Preparation of stable Pd nanoparticles with tunable size for multiple immunolabeling in biomedicine
Author(s)	Šlouf, Miroslav (UMCH-V)_{RID, ORCID} Pavlova, Ewa (UMCH-V)_RID Bhardwaj, Monika (UMCH-V) Pleštil, Josef (UMCH-V)_RID Onderková, H. (CZ) Philimonenko, Anatoly (UMG-J) Hozák, Pavel (UMG-J)_{RID, ORCID}
Source Title	Materials Letters. - : ELSEVIER SCIENCE BV - ISSN 0167-577X Roč. 65, č. 8 (2011), s. 1197-1200
Number of pages	4 s.
Language	eng - English
Country	NL - Netherlands
Keywords	palladium nanoparticles ; nanocrystalline materials ; immunolabeling
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	KAN200520704 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	GAP205/10/0348 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z40500505 - UMCH-V (2005-2011)
	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000288928500017
DOI	10.1016/j.matlet.2011.01.046
Annotation	Stable Pd nanoparticles (PdNP) with a tunable size (3-15 nm) were synthesized by controlled chemical reduction of PdCl2 with sodium citrate in water. The morphology of PdNP was characterized by transmission electron microscopy, while their stability in solution was verified by quasi-elastic light scattering and small-angle X-ray scattering. Intensive stirring of reacting mixture played a vital role in achieving reproducible particle sizes. Controlled changes of pH and initial concentrations were employed in fine-tuning particle size distributions. Finally, 10 nm PdNP were conjugated with goat anti-mouse IgG antibody as proved by electrophoresis (SOS-PAGE) and used for ultrastructural immunolabeling, which confirmed suitability of PdNP for multiple immunolabeling in biomedicine.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2012

Number of the records: 1