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Glycerol-3-phosphate Dehydrogenase Expression and Oxygen Consumption in Liver Mitochondria of Female and Male Rats with Chronic Alteration of Thyroid Status
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SYSNO ASEP 0359114 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Glycerol-3-phosphate Dehydrogenase Expression and Oxygen Consumption in Liver Mitochondria of Female and Male Rats with Chronic Alteration of Thyroid Status Author(s) Rauchová, Hana (FGU-C) RID, ORCID
Mráček, Tomáš (FGU-C) RID, ORCID
Novák, Petr (FGU-C)
Vokurková, Martina (FGU-C) RID
Soukup, Tomáš (FGU-C) RIDSource Title Hormone and Metabolic Research - ISSN 0018-5043
Roč. 43, č. 1 (2011), s. 43-47Number of pages 5 s. Language eng - English Country DE - Germany Keywords chronic administration ; triiodothyronine ; methimazole ; sex difference ; idebenone Subject RIV ED - Physiology R&D Projects GA303/09/0570 GA ČR - Czech Science Foundation (CSF) GA304/08/0256 GA ČR - Czech Science Foundation (CSF) GPP303/10/P227 GA ČR - Czech Science Foundation (CSF) 1M0510 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50110509 - FGU-C (2005-2011) UT WOS 000285914900008 DOI 10.1055/s-0030-1265220 Annotation In our chronic experiments the activity and protein amount of glycerol-3-phosphate dehydrogenase (GPDH) in mitochondria isolated from the liver of adult male and female inbred Lewis strain euthyroid (EU), hyperthyroid (TH) and hypothyroid (HY) rats were analyzed by biochemical and Western blot methods. The TH status led to a significant increase and HY status to a significant decrease of enzyme activity and protein amount in both male and female animals. These changes were, however, more pronounced in females. Measurements of serum thyroid hormone levels and analysis of anatomical parameters confirmed that our procedures inducting the TH and HY states are efficient and reliable and that determination of GPDH can serve as an additional criterion for the evaluation of the thyroid hormone status Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2012
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