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The osmotolerant fructophilic yeast Zygosaccharomyces rouxii employs two plasma-membrane fructose uptake systems belonging to a new family of yeast sugar transporters
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SYSNO ASEP 0358896 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The osmotolerant fructophilic yeast Zygosaccharomyces rouxii employs two plasma-membrane fructose uptake systems belonging to a new family of yeast sugar transporters Author(s) Leandro, Maria José (FGU-C)
Sychrová, Hana (FGU-C) RID, ORCID
Prista, C. (PT)
Loureiro-Dias, M.C. (PT)Source Title Microbiology. - : Microbiology Society - ISSN 1350-0872
Roč. 157, č. 2 (2011), s. 601-608Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords Zygosaccharomyces rouxii ; fructose transporter ; heterologous expression ; Saccharomyces cerevisiae Subject RIV EE - Microbiology, Virology R&D Projects LC531 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP503/10/0307 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50110509 - FGU-C (2005-2011) UT WOS 000288483200029 DOI 10.1099/mic.0.044446-0 Annotation We have identified two genes encoding putative fructose transporters in the Z. rouxii CBS 732 genome. Heterologous expression of the two Z. rouxii ORFs in a S. cerevisiae strain lacking its own hexose transporters (hxt-null) and subsequent kinetic analysis of sugar transport showed that both proteins are functionally expressed at the plasma-membrane, ZrFfz1 being a high capacity fructose-specific facilitator and ZrFfz2 a facilitator transporting glucose and fructose with similar capacity and affinity Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2012
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