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Campylobacter troglodytis sp. nov., isolated from feces of human-habituated wild chimpanzees (Pan troglodytes schweinfurthii) in Tanzania

SYSNO ASEP	0358877
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Campylobacter troglodytis sp. nov., isolated from feces of human-habituated wild chimpanzees (Pan troglodytes schweinfurthii) in Tanzania
Author(s)	Kaur, T. (US) Singh, J. (US) Huffman, M. A. (JP) Petrželková, Klára Judita (UBO-W)_{RID, SAI, ORCID} Taylor, N. S. (US) Xu, S. (US) Dewhirst, F. E. (US) Paster, B. J. (US) Debruyne, L. (BE) Vandamme, P. (BE) Fox, J. G. (US)
Number of authors	11
Source Title	Applied and Environmental Microbiology. - : American Society for Microbiology - ISSN 0099-2240 Roč. 77, č. 7 (2011), s. 2366-2373
Number of pages	8 s.
Language	eng - English
Country	US - United States
Keywords	Mahale Mountains ; intestinal parasites ; genetic relatedness
Subject RIV	EG - Zoology
CEZ	AV0Z60930519 - UBO-W (2005-2011)
UT WOS	000288855500022
EID SCOPUS	79953273357
DOI	10.1128/AEM.01840-09
Annotation	The transmission of simian immunodeficiency and Ebola viruses to humans in recent years has heightened awareness of the public health significance of zoonotic diseases of primate origin, particularly from chimpanzees. In this study, we analyzed 71 fecal samples collected from 2 different wild chimpanzee (Pan troglodytes) populations with different histories in relation to their proximity to humans. Campylobacter spp. were detected by culture in 19/56 (34%) group 1 (human habituated for research and tourism purposes at Mahale Mountains National Park) and 0/15 (0%) group 2 (not human habituated but propagated from an introduced population released from captivity over 30 years ago at Rubondo Island National Park) chimpanzees, respectively. Using 16S rRNA gene sequencing, all isolates were virtually identical (at most a single base difference), and the chimpanzee isolates were most closely related to Campylobacter helveticus and Campylobacter upsaliensis (94.7% and 95.9% similarity, respectively). Whole-cell protein profiling, amplified fragment length polymorphism analysis of genomic DNA, hsp60 sequence analysis, and determination of the mol% G+C content revealed two subgroups among the chimpanzee isolates. DNA-DNA hybridization experiments confirmed that both subgroups represented distinct genomic species. In the absence of differential biochemical characteristics and morphology and identical 16S rRNA gene sequences, we propose to classify all isolates into a single novel nomenspecies, Campylobacter troglodytis, with strain MIT 05-9149 as the type strain; strain MIT 05-9157 is suggested as the reference strain for the second C. troglodytis genomovar. Further studies are required to determine whether the organism is pathogenic to chimpanzees and whether this novel Campylobacter colonizes humans and causes enteric disease.
Workplace	Institute of Vertebrate Biology
Contact	Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524
Year of Publishing	2012

Number of the records: 1