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Complex I (NADH:ubiquinone oxidoreductase) is active in but non-essential for procyclic Trypanosoma brucei

SYSNO ASEP	0358455
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Complex I (NADH:ubiquinone oxidoreductase) is active in but non-essential for procyclic Trypanosoma brucei
Author(s)	Verner, Zdeněk (BC-A) Čermáková, P. (SK) Škodová, Ingrid (BC-A)_ORCID Kriegová, Eva (BC-A)_SAI Horváth, A. (SK) Lukeš, Julius (BC-A)_{RID, ORCID}
Source Title	Molecular and Biochemical Parasitology. - : Elsevier - ISSN 0166-6851 Roč. 175, č. 2 (2011), s. 196-200
Number of pages	5 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Trypanosoma ; Mitochondrion ; Respiration ; Complex I
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA204/09/1667 GA ČR - Czech Science Foundation (CSF)
	GD206/09/H026 GA ČR - Czech Science Foundation (CSF)
	2B06129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LC07032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000286556100013
DOI	10.1016/j.molbiopara.2010.11.003
Annotation	The requirement of complex I (NADH:ubiquionone oxidoreductase) for respiration in Trypanosoma brucei is controversial. Recent identification of its homologues in mitochondrial proteome resolved a question of its presence or absence. However, with one exception, no data have been available concerning the function(s) of complex I. Here we present a functional RNAi study of 3 (NUBM, NUKM, NUEM) putative subunits of this complex. Although no changes were detected in growth, mitochondrial membrane potential or reactive oxygen species production in cell lines depleted for target transcript, the NUBM and NUKM RNAi knock-downs showed decreased specific NADH:ubiquinone oxidoreductase activity. Moreover, glycerol gradients revealed the presence of two distinct peaks of NADH dehydrogenase activity, with shifted sensitivity to inhibitors of complex I upon RNAi induction. Thus complex I is not only present in the procyclic stage, but it does participate in electron transport chain.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2012

Number of the records: 1