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Development of multiplex microsatellite sets for noninvasive population genetic study of the endangered Tatra chamois

SYSNO ASEP	0358425
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Development of multiplex microsatellite sets for noninvasive population genetic study of the endangered Tatra chamois
Author(s)	Zemanová, Barbora (UBO-W)_{ORCID, SAI, RID} Hájková, Petra (UBO-W)_{RID, ORCID} Bryja, Josef (UBO-W)_{RID, SAI, ORCID} Zima Jr., Jan (UBO-W) Hájková, Andrea (UBO-W) Zima, Jan (UBO-W)_{RID, SAI}
Number of authors	6
Source Title	Folia zoologica. - : Ústav biologie obratlovců AV ČR, v. v. i. - ISSN 0139-7893 Roč. 60, č. 1 (2011), s. 70-80
Number of pages	11 s.
Language	eng - English
Country	CZ - Czech Republic
Keywords	noninvasive genetic sampling ; amplification success rate ; ungulates
Subject RIV	EG - Zoology
R&D Projects	IAA600930609 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z60930519 - UBO-W (2005-2011)
UT WOS	000289010400011
EID SCOPUS	79952821649
Annotation	The only autochthonous population of Tatra chamois (Rupicapra rupicapra tatrica) occurs in the Tatra Mountains (N Slovakia and S Poland). Another population has been introduced to the Low Tatra Mts., while Alpine chamois (R. r. rupicapra) has been introduced to the neighbouring mountain ranges, Veľká Fatra and Slovenský raj. All these populations have undergone intensive bottlenecks, which could result in prevailing monomorphism of available microsatellite markers. We tested 65 markers previously used in chamois or other Caprinae species, from which 20 most suitable loci for noninvasive genetic study of the Tatra chamois were used for optimisation of three multiplex sets. Low genetic diversity was observed in the Tatra chamois populations while slightly higher values were obtained for Alpine chamois population in Slovenský raj. We subsequently assessed the amplification success rate for noninvasively obtained samples (faeces).
Workplace	Institute of Vertebrate Biology
Contact	Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524
Year of Publishing	2012
Electronic address	http://www.ivb.cz/folia/60/1/11_2011.pdf

Number of the records: 1