Number of the records: 1
The effects of membrane compartmentalization of csk on TCR signaling
- 1.
SYSNO ASEP 0358089 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The effects of membrane compartmentalization of csk on TCR signaling Author(s) Otáhal, Pavel (UMG-J) RID
Pata, Supansa (UMG-J)
Angelisová, Pavla (UMG-J) RID
Hořejší, Václav (UMG-J) RID
Brdička, Tomáš (UMG-J) RIDSource Title Biochimica et biophysica acta - ISSN 0006-3002
Roč. 1813, č. 2 (2010), s. 367-376Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords T-cell activation ; lipid rafts ; CBP Subject RIV EB - Genetics ; Molecular Biology R&D Projects GEMEM/09/E011 GA ČR - Czech Science Foundation (CSF) 1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000287470800010 DOI 10.1016/j.bbamcr.2010.12.003 Annotation The TCR signal transduction is initiated by the activation of Src-family kinases (SFK) which phosphorylate Immunoreceptor tyrosine-based activation motifs (ITAM) present in the intracellular parts of the T-cell receptor (TCR) signaling subunits. Numerous data suggest that after stimulation TCR interacts with membrane rafts and thus it gains access to SFK and other important molecules involved in signal transduction. One of the key questions is how SFK access TCR and what is the importance of non-raft and membrane raft-associated SFK for the initiation and maintenance of the TCR signaling. To answer this question we targeted a negative regulator of SFK, C-terminal Src kinase (Csk) to membrane rafts, recently described "heavy rafts" or non-raft membrane. Our data show that only Csk targeted into "classical" raft but not to "heavy raft" or non-raft membrane effectively inhibits TCR signaling, demonstrating the critical role of membrane raft-associated SFK in this process. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
Number of the records: 1