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Pre-sorting endosomal transport of the GPI-anchored protein, CD59, is regulated by EHD1

SYSNO ASEP	0358085
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Pre-sorting endosomal transport of the GPI-anchored protein, CD59, is regulated by EHD1
Author(s)	Cai, B. (US) Katafiasz, D. (US) Hořejší, Václav (UMG-J)_RID Naslavsky, N. (US)
Source Title	Traffic. - : Wiley - ISSN 1398-9219 Roč. 12, č. 1 (2011), s. 102-120
Number of pages	19 s.
Language	eng - English
Country	DK - Denmark
Keywords	canine kidney cells ; recycling compartment ; receptor
Subject RIV	EB - Genetics ; Molecular Biology
CEZ	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000285206500010
DOI	10.1111/j.1600-0854.2010.01135.x
Annotation	The potential role of EHD1 in regulating the family of glycosylphosphatidylinositol-anchored proteins (GPI-APs) has not been determined. Here we demonstrate a novel role for EHD1 in regulating the trafficking of CD59, an endogenous GPI-AP, at early stages of trafficking through the endocytic pathway. EHD1 displays significant colocalization with newly internalized CD59. Upon EHD1 depletion, there is a rapid Rab5-independent coalescence of CD59 in the ERC region. However, expression of an active Arf6 mutant (Q67L), which traps internalized pre-sorting endosomal cargo in phosphatidylinositol(4,5)-bisphosphate enriched vacuoles, prevents this coalescence. It is of interest that sustained PKC activation leads to a similar coalescence of CD59 at the ERC, and treatment of EHD1-depleted cells with a PKC inhibitor (Go6976) blocked this rapid relocation of CD59. However, unlike sustained PKC activation, EHD1 depletion does not induce the translocation of PKC alpha to ERC.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2011

Number of the records: 1