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LC-ESI-MS/MS method for oxidative stress multimarker screening in the exhaled breath condensate of asbestosis/silicosis patients
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SYSNO ASEP 0357968 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title LC-ESI-MS/MS method for oxidative stress multimarker screening in the exhaled breath condensate of asbestosis/silicosis patients Author(s) Syslová, K. (CZ)
Kačer, P. (CZ)
Kuzma, Marek (MBU-M) ORCID, RID
Pankrácová, A. (CZ)
Fenclová, Z. (CZ)
Vlčková, Š. (CZ)
Lebedová, J. (CZ)
Pelclová, D. (CZ)Source Title Journal of Breath Research. - : Institute of Physics Publishing - ISSN 1752-7155
Roč. 4, č. 1 (2010), s. 1-8Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords 8-ISOPROSTANE ; ASBESTOS ; MARKERS Subject RIV EE - Microbiology, Virology R&D Projects NR9338 GA MZd - Ministry of Health (MZ) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000283129400008 DOI 10.1088/1752-7155/4/1/017104 Annotation The sensitive assay method was developed for a parallel, rapid and precise determination of the most prominent oxidative stress biomarkers: 8-iso-prostaglandin F2α a lipid oxidation biomarker, o-tyrosine an amino acid oxidation biomarker and 8-hydroxy-2_-deoxy-guanosine a nucleic acid oxidation biomarker. The method consisted of a pre-treatment part, freeze drtiny (lyophilization), serving the purpose of biomarkers concentration from the exhaled breath condensate and detection method LC-ESI-MS/MS, where the selected reaction-monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
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