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Lignification and related parameters in copper-exposed Matricaria chamomilla roots: Role of H2O2 and NO in this process
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SYSNO ASEP 0357896 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Lignification and related parameters in copper-exposed Matricaria chamomilla roots: Role of H2O2 and NO in this process Author(s) Kováčik, J. (SK)
Grúz, Jiří (UEB-Q) RID, ORCID
Klejdus, B. (CZ)
Štork, F. (SK)
Marchiosi, R. (BR)
Ferrarese-Filho, O. (BR)Source Title Plant Science. - : Elsevier - ISSN 0168-9452
Roč. 179, č. 4 (2010), s. 383-389Number of pages 7 s. Language eng - English Country IE - Ireland Keywords Heavy metals ; Oxidative stress ; Phenols Subject RIV EF - Botanics CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000281219500012 DOI 10.1016/j.plantsci.2010.06.014 Annotation Despite many investigations of the role of ROS and NO in plant metabolism, their involvement in lignification is still poorly understood. We tested the regulatory role of hydrogen peroxide (scavenged by dithiothreitol, DTT) and nitric oxide (scavenged by 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxy1-3-oxide, PTIO) using chamomile roots exposed to Cu for 24 h. The application of DTT and PTIO led to a compensatory increase in NO and H2O2, respectively. DTT prevented Cu-induced lignin accumulation concomitant with a decrease in H2O2 content and the activities of guaiacol- and ascorbate peroxidases. Cinnamyl alcohol dehydrogenase (CAD) and polyphenol oxidase (PPO) also decreased. DTT stimulated accumulation of total soluble phenols and phenolic acids in all three fractions investigated. The opposite trend was recorded in PTIO-treated roots. Within the free amino acids, proline and phenylalanine increased in response to DTT application. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
Number of the records: 1