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Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta)
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SYSNO ASEP 0357655 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta) Author(s) Kvíderová, Jana (BU-J) RID, ORCID Number of authors 1 Source Title Environmental Toxicology - ISSN 1520-4081
Roč. 25, č. 6 (2010), s. 554-562Number of pages 10 s. Language eng - English Country US - United States Keywords bioassay ; variable chlorophyll fluorescence ; Chlorella kessleri Subject RIV EF - Botanics R&D Projects 1M0571 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z60050516 - BU-J (2005-2011) UT WOS 000284683800003 DOI 10.1002/tox.20516 Annotation Three methods of algal assays with green alga Chlorella kessleri - the standard assay, microassay, and the proposed fluorescence assay - were compared from the point of view of reliability of EC50 detection, the minimum required time for the detection, sensitivity of individual measurement, i.e. at which cell density the particular assay can be used for EC50 estimation, and the time stability of the EC50 values. No significant differences in EC50 between the assay type and the inoculation methods were observed with the exception of the significant difference between ECc50-72 in the standard assay and ECr50 in the microassay in the standard inoculation. The ECf50 derived from variable fluorescence measurement values correspond to EC50 values derived from the growth rates. Fluorescence measurement revealed the toxic effect after 24 h of exposure (24 h before the other bioassays) at lower cell density (50%). Workplace Institute of Botany Contact Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Year of Publishing 2011
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