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Defence responses induced in embryogenic cultures of Norway spruce by two fractions of Gremmeniella abietina mycelia
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SYSNO ASEP 0357022 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Defence responses induced in embryogenic cultures of Norway spruce by two fractions of Gremmeniella abietina mycelia Author(s) Cvikrová, Milena (UEB-Q) RID, ORCID
Malá, J. (CZ)
Hrubcová, Marie (UEB-Q)
Martincová, Olga (UEB-Q)
Cvrčková, H. (CZ)
Lipavská, H. (CZ)Source Title Forest Pathology. - : Wiley - ISSN 1437-4781
Roč. 40, č. 6 (2010), s. 467-484Number of pages 18 s. Language eng - English Country DE - Germany Keywords defence response ; Norway spruce ; Gremmeniella abietina Subject RIV GK - Forestry R&D Projects QH82303 GA MZe - Ministry of Agriculture (MZe) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000284892200001 DOI 10.1111/j.1439-0329.2009.00622.x Annotation We have examined defence responses in embryogenic cell suspension cultures of Norway spruce elicited by intracellular protein and wall fractions (PF and WF) prepared from mycelia of the fungus Gremmeniella abietina, focusing on changes in levels of phenolics, polyamines and activities of enzymes that catalyse important steps in their metabolism: phenylalanine ammonia-lyase and diamine oxidase. Treatment with both preparations induced increases in the spruce cells total contents of phenolic acids and polyamines but they responded more rapidly to WF than to PF. The contents of soluble and cell wall-bound phenolic acids and soluble conjugates of putrescine started to increase relative to controls (C) within 4 h of the addition of the WF preparation. These changes correlated with the induction of PAL activity in spruce cells at 4 and 8 h following the addition of WF and PF preparations. Increases in DAO activity were also detected after 4 h exposure to WF, and after 24 h exposure to PF. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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