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Differential expression of non-coding RNAs and continuous evolution of the X chromosome in testicular transcriptome of two mouse species
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SYSNO ASEP 0356977 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Differential expression of non-coding RNAs and continuous evolution of the X chromosome in testicular transcriptome of two mouse species Author(s) Homolka, David (UMG-J)
Ivánek, Robert (UMG-J)
Forejt, Jiří (UMG-J) RID, ORCID
Jansa, Petr (UMG-J) RIDSource Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 6, č. 6 (2011), e17198-e17198Number of pages 14 s. Language eng - English Country US - United States Keywords spermatogenesis ; tiling array ; expression profiles Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA301/07/1383 GA ČR - Czech Science Foundation (CSF) 1M0520 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000287367600041 DOI 10.1371/journal.pone.0017198 Annotation Tight regulation of testicular gene expression is a prerequisite for male reproductive success, while differentiation of gene activity in spermatogenesis is important during speciation. We compared testicular transcriptomes of two closely related mouse species, Mus musculus and Mus spretus. We analyzed testicular expression using tiling arrays overlapping Chromosomes 2, X, Y and mitochondrial genome. An excess of differentially regulated non-coding RNAs was found on Chromosome 2 including the intronic antisense RNAs, intergenic RNAs and premature piRNAs. This indicates the role of non-coding RNAs in species-specific regulation of spermatogenesis. A striking difference was found in the expression of X-linked G6pdx gene, the parental gene of the autosomal retrogene G6pd2. The postmeiotic expression of G6pdx in Mus spretus points towards the continuous evolution of X-chromosome silencing and provides an example of expression change accompanying the out-of-the X-chromosomal retroposition. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
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