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The role of actin isoforms in somatic embryogenesis in Norway spruce
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SYSNO ASEP 0356952 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The role of actin isoforms in somatic embryogenesis in Norway spruce Author(s) Schwarzerová, K. (CZ)
Vondráková, Zuzana (UEB-Q) RID, ORCID
Fischer, L. (CZ)
Boříková, P. (CZ)
Bellinvia, E. (CZ)
Eliášová, Kateřina (UEB-Q) RID, ORCID
Havelková, L. (CZ)
Fišerová, J. (CZ)
Vágner, Martin (UEB-Q) RID, ORCID
Opatrný, Z. (CZ)Source Title BMC Plant Biology. - : BioMed Central - ISSN 1471-2229
Roč. 10, č. 89 (2010), s. 1-13Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords PROGRAMMED CELL-DEATH ; GENE FAMILY ; F-ACTIN Subject RIV ED - Physiology R&D Projects LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) OC 158 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ME 668 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000279956500001 DOI 10.1186/1471-2229-10-89 Annotation Application of low doses of latrunculin B (lat B) during the maturation of somatic embryos predominantly killed suspensor cells while leaving the cells in meristematic centres alive, indicating differential sensitivity of actin in the two cell types. The treatment resulted in faster development of advanced embryos into mature somatic embryos and elimination of insufficiently developed ones. We analysed the composition of actin isoforms in the culture and isolated four spruce actin genes. Analysis of their expression during embryo maturation revealed that one actin isoform was expressed constitutively in both cell types, whereas three actin isoforms were expressed predominantly in suspensor cells and their expression declined during the maturation. The expression decline was enhanced by Lat B treatment. Sequence analysis revealed amino-acid substitutions in the Lat B-binding site in one of the suspensor-specific actin isoforms, which may result in a different binding affinity for Lat B. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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