Facile end-labeling of RNA with electroactive Os(VI) complexes

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SYSNO ASEP	0356812
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Facile end-labeling of RNA with electroactive Os(VI) complexes
Author(s)	Trefulka, Mojmír (BFU-R)_{RID, ORCID} Bartošík, Martin (BFU-R)_RID Paleček, Emil (BFU-R)_{RID, ORCID}
Number of authors	3
Source Title	Electrochemistry Communications. - : Elsevier - ISSN 1388-2481 Roč. 12, č. 12 (2010), s. 1760-1763
Number of pages	4 s.
Language	eng - English
Country	US - United States
Keywords	RNA end-labeling ; voltammetry ; osmium complexes
Subject RIV	BO - Biophysics
R&D Projects	GPP301/10/P548 GA ČR - Czech Science Foundation (CSF)
	ME09038 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LC06035 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50040507 - BFU-R (2005-2011)
	AV0Z50040702 - BFU-R (2007-2013)
UT WOS	000285904700024
DOI	10.1016/j.elecom.2010.10.016
Annotation	Ribose at the 3'-end of oligonucleotides (oligos) selectively modified by Os(VI)2,2'-bipyridine (bipy) produced two CV redox couples at pyrolytic graphite electrode. Using square wave voltammetry (SWV) 22-mer oligos can be detected down to 250 nM. At mercury electrodes the Os(VI)bipy-oligo adducts produced an electrocatalytic peak at ~-1.2 V allowing their determination down to picomolar concentrations.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2011

Number of the records: 1