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Cancer Drug-Resistance and a Look at Specific Proteins: Rho GDP-Dissociation Inhibitor 2, Y-Box Binding Protein 1, and HSP70/90 Organizing Protein in Proteomics Clinical Application

SYSNO ASEP	0356672
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Cancer Drug-Resistance and a Look at Specific Proteins: Rho GDP-Dissociation Inhibitor 2, Y-Box Binding Protein 1, and HSP70/90 Organizing Protein in Proteomics Clinical Application
Author(s)	Skalníková, Helena (UZFG-Y)_{RID, ORCID} Martinková, Jiřina (UZFG-Y) Hrabáková, Rita (UZFG-Y)_{RID, ORCID} Halada, Petr (MBU-M)_{RID, ORCID} Dziechciarková, M. (CZ) Hajdúch, M. (CZ) Gadher, S. J. (CH) Hammar, A. (SE) Enetoft, D. (SE) Ekefjard, A. (SE) Forsstrom-Olsson, O. (SE) Kovářová, Hana (UZFG-Y)_{RID, ORCID}
Source Title	Journal of Proteome Research. - : American Chemical Society - ISSN 1535-3893 Roč. 10, č. 2 (2011), s. 404-415
Number of pages	12 s.
Language	eng - English
Country	US - United States
Keywords	cancer ; anti-cancer drugs ; drug resistance
Subject RIV	CE - Biochemistry
R&D Projects	LC07017 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA301/08/1649 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000286868500004
DOI	10.1021/pr100468w
Annotation	Resistance to anti-cancer drugs is a well recognized problem and very often it is responsible for failure of the cancer treatment. In this study, the proteome alterations associated with the development of acquired resistance to cyclin-depedent kinases inhibitor bohemine, a promising anti-cancer drug, were analyzed with the primary aim of identifying potential targets of resistance within the cell that could pave a way to selective elimination of specific resistant cell types. A model of parental susceptible CEM T-lymphoblastic leukemia cells and its resistant counterpart CEM-BOH was used and advanced 2-D liquid chromatography was applied to fractionate cellular proteins. Differentially expressed identified proteins were further verified using immunoblotting and immunohistochemistry. Our study has revealed that Rho GDP-dissociation inhibitor 2, Y-box binding protein 1, and the HSP70/90 organizing protein have a critical role to play in resistance to cyclin-depedent kinases inhibitor. The results indicated not only that quantitative protein changes play an important role in drug-resistance, but also that there are various other parameters such as truncation, post-translational modification(s), and subcellular localization of selected proteins. Furthermore, these proteins were validated for their roles in drug resistance using different cell lines resistant to diverse representatives of anti-cancer drugs such as vincristine and daunorubicin
Workplace	Institute of Animal Physiology and Genetics
Contact	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Year of Publishing	2011

Number of the records: 1