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Systematically fragmented genes in a multipartite mitochondrial genome
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SYSNO ASEP 0356039 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Systematically fragmented genes in a multipartite mitochondrial genome Author(s) Vlček, Čestmír (UMG-J) RID
Marande, W. (CA)
Teijeiro, S. (CA)
Lukeš, Julius (BC-A) RID, ORCID
Burger, G. (CA)Source Title Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 39, č. 3 (2011), s. 979-988Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords mtDNA genome ; gene fragmentation ; RNA editing Subject RIV EB - Genetics ; Molecular Biology R&D Projects 1M0520 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA204/09/1667 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000287257500024 DOI 10.1093/nar/gkq883 Annotation The most bizarre mitochondrial DNA (mtDNA) is that of the euglenozoan eukaryote Diplonema papillatum. The genome consists of numerous small circular chromosomes none of which appears to encode a complete gene. We examined how many genes are encoded by Diplonema mtDNA and whether all are fragmented and their transcripts trans-spliced. By employing most sensitive protein profile search algorithms and comparing genomic with cDNA sequence, we recognize a total of 11 typical mitochondrial genes. The 10 protein-coding genes are systematically chopped up into three to 12 modules of 60-350 bp length. The corresponding mRNAs are all trans-spliced. Our results open new intriguing questions about the biochemistry and evolution of mitochondrial trans-splicing in Diplonema. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2013
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