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Toward the crystallization of photosystem II core complex from Pisum sativum L
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SYSNO ASEP 0355837 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Toward the crystallization of photosystem II core complex from Pisum sativum L Author(s) Prudnikova, T. (CZ)
Gavira, J. A. (ES)
Řezáčová, Pavlína (UMG-J) RID
Molina, E.P. (ES)
Hunalová, Ivana (BC-A)
Sviridova, E. (CZ)
Schmidt, V. (CZ)
Kohoutová, J. (CZ)
Kutý, Michal (UEK-B)
Kaftan, D. (CZ)
Vácha, F. (CZ)
Garcia-Ruiz, J. M. (ES)
Kutá-Smatanová, Ivana (UEK-B) RIDSource Title Crystal Growth & Design. - : American Chemical Society - ISSN 1528-7483
Roč. 10, č. 8 (2010), s. 3391-3396Number of pages 6 s. Language eng - English Country US - United States Keywords crystal-structure ; cyanobacterial ; elongatus Subject RIV EB - Genetics ; Molecular Biology R&D Projects LC06010 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) AV0Z50510513 - UMBR-M, BC-A (2005-2011) AV0Z60870520 - UEK-B (2005-2011) AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000280471700017 DOI 10.1021/cg901593x Annotation The crystallization of the higher plant similar to 1 gigadalton homodimeric lipid-pigment-protein complex of photosystem II constitutes a challenging undertaking that has not been crowned with success to date. In the continuation of our previous efforts (Kuta-Smatanova et al. Photosyn. Res. 2006, 90, 255-259) that had provided needle and plate crystals of plant photosystem II for the first time, we report on a study to crystallize three-dimensional crystals of the photosystem 11 from Pisum sativum L. suitable for X-ray analysis. In our screening, we optimized temperature, supersaturation rate, and concentrations of detergents, buffers, salts, and organic additives. We used a sitting and hanging drop vapor-diffusion method, microbatch under oil and counter-diffusion techniques, and direct and cross-influenced procedures. We have identified that the major limiting factors for the growth of crystals are the photosystem II stability and requirement of indispensable divalent cations. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
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