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A method for preserving ultrastructural properties of mitotic cells for subsequent immunogold labeling using low-temperature embedding in LR White resin

SYSNO ASEP	0355821
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	A method for preserving ultrastructural properties of mitotic cells for subsequent immunogold labeling using low-temperature embedding in LR White resin
Author(s)	Sobol, Margaryta (UMG-J)_RID Nebesářová, Jana (BC-A)_{RID, ORCID} Hozák, Pavel (UMG-J)_{RID, ORCID}
Source Title	Histochemistry and Cell Biology. - : Springer - ISSN 0948-6143 Roč. 135, č. 1 (2011), s. 103-110
Number of pages	8 s.
Language	eng - English
Country	DE - Germany
Keywords	Mitotic cells ; High-pressure freezing ; Histochemistry
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	KAN200520704 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	2B06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50520514 - UMG-J (2005-2011)
	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000286794000011
DOI	10.1007/s00418-010-0771-1
Annotation	The best available approach of biological sample preparation for transmission electron microscopy currently includes cryoimmobilization by high-pressure freezing (HPF) followed by freeze-substitution (FS). This method has been well established for interphase cells; however, a reliable and easy procedure is still missing for mitotic cells especially because of their fragility and sensitivity to treatments. Here, we present a fast and effective method for HPF/automated FS and LR White embedding of mitotic cells which allows for their controlled and reproducible quality processing. It should be useful in various ultrastructural studies on mitotic cells especially in combination with immunocytochemistry.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2011

Number of the records: 1