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Automated counting of yeast colonies using the Fast Radial Transform algorithm
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SYSNO ASEP 0355696 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Automated counting of yeast colonies using the Fast Radial Transform algorithm Author(s) Schier, Jan (UTIA-B) RID
Kovář, Bohumil (UTIA-B) RIDSource Title BIOSTEC 2011 4th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOINFORMATICS 2011 Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms. - Lisabon : SciTePress, 2011 / Pellegrini Marco ; Fred Ana ; Filipe Joaquim ; Gamboa Hugo - ISBN 978-989-8425-36-2 Pages s. 22-27 Number of pages 6 s. Publication form CD-ROM - CD-ROM Action BIOINFORMATICS 2011 International Conference on Bioinformatics Models, Methods and Algorithms Event date 26.01.2011-29.1.2011 VEvent location Řím Country IT - Italy Event type WRD Language eng - English Country PT - Portugal Keywords Colony counter ; Yeast colonies ; Image segmentation ; Fast radial transform Subject RIV IN - Informatics, Computer Science R&D Projects 1M0567 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z10750506 - UTIA-B (2005-2011) UT WOS 000308455800009 DOI 10.5220/0003129000220027 Annotation A method for counting yeast colonies in images of Petri dishes, based on the Fast Radial Transform by Loy and Zelinsky, is introduced and evaluated in the paper. Images, produced by a general-purpose imaging setup, are considered. The performance of the method has been evaluated with a test set of 245 images. The images typically contained between 10 and 70 colonies per dish, with relative coverage of the dish less than 10% of the area. The average counting error (missed colonies) on this set was under 4%. A tool, implementing the method, has been developed in Matlab. The tool provides a batch mode for processing of larger image sets prepared beforehand in the darkroom and it automates the process of counting as much as possible. It is available for download or can be requested from the authors of the paper, in both cases free on charge. Workplace Institute of Information Theory and Automation Contact Markéta Votavová, votavova@utia.cas.cz, Tel.: 266 052 201. Year of Publishing 2011
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