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Equilibrative nucleoside transporter 2 regulates olfactory memory and synaptic function in Drosophila
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SYSNO ASEP 0355637 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Equilibrative nucleoside transporter 2 regulates olfactory memory and synaptic function in Drosophila Author(s) Knight, D. (CA)
Harvey, P. J. (CA)
Iliadi, K. G. (CA)
Klose, M. K. (CA)
Iliadi, N. (CA)
Doleželová, Eva (BC-A) RID
Charlton, M. P. (CA)
Žurovec, Michal (BC-A) RID, ORCID
Boulianne, G. L. (CA)Number of authors 9 Source Title Journal of Neuroscience. - : Society for Neuroscience - ISSN 0270-6474
Roč. 30, č. 14 (2010), s. 5047-5057Number of pages 11 s. Language eng - English Country US - United States Keywords Drosophila Subject RIV ED - Physiology R&D Projects LC06077 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50070508 - ENTU-I, BC-A (2005-2011) UT WOS 000276414800025 DOI 10.1523/JNEUROSCI.6241-09.2010 Annotation Nucleoside transporters are evolutionarily conserved proteins that are essential for normal cellular function. In the present study, we examined the role of equilibrative nucleoside transporter 2 (ent2) in Drosophila. Null mutants of ent2 are lethal during late larval/early pupal stages, indicating that ent2 is essential for normal development. Hypomorphic mutant alleles of ent2, however, are viable and exhibit reduced associative learning. We additionally used RNA interference to knock down ent2 expression in specific regions of the CNS and show that ent2 is required in the alpha/beta lobes of the mushroom bodies and the antennal lobes. To determine whether the observed behavioral defects are attributable to defects in synaptic transmission, we examined transmitter release at the larval neuromuscular junction (NMJ). Excitatory junction potentials were significantly elevated in ent2 mutants, whereas paired-pulse plasticity was reduced. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2011
Number of the records: 1