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Characterization of Lymphocyte Subsets in Patients with Common Variable Immunodeficiency Reveals Subsets of Naive Human B Cells Marked by CD24 Expression
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SYSNO ASEP 0355461 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Characterization of Lymphocyte Subsets in Patients with Common Variable Immunodeficiency Reveals Subsets of Naive Human B Cells Marked by CD24 Expression Author(s) Vlková, M. (CZ)
Froňková, E. (CZ)
Kanderová, V. (CZ)
Janda, A. (CZ)
Růžičková, Šárka (BTO-N) RID
Litzman, J. (CZ)
Šedivá, A. (CZ)
Kalina, T. (CZ)Source Title Journal of Immunology. - : American Association of Immunologists - ISSN 0022-1767
Roč. 185, č. 11 (2010), s. 6431-6438Number of pages 8 s. Language eng - English Country US - United States Keywords B lymphocyte immunophenotyping ; naive B cells ; common variable immunodeficiency Subject RIV EC - Immunology CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000284311500007 DOI 10.4049/jimmunol.0903876 Annotation B cells from 48 patients with CVID and 49 healthy controls were analyzed by polychromatic flow cytometry to examine phenotype. We have described two groups of patients with normal (CVID-21norm) or increased (CVID-21lo) proportions of CD27(neg)CD21(neg)CD38(neg) B cells. Upon further analyses, we found two discrete subpopulations of this subset based on the expression of CD24. The B cell subsets showed a markedly increased proliferation in CVID-21lo patients as compared with healthy controls, suggesting developmental arrest rather than increased bone marrow output. In additon, two different subpopulations based on IgM and CD24 expression were found corresponding to follicular (FO) I and FO II cells previously described in mice. We propose that the CD27(neg)CD21(neg)CD38(neg) B cells increased in some of the CVID patients originate from the two FO subsets after loss of CD21 expression Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2011
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