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Neoplastic progression of the human breast cancer cell line G3S1 is associated with elevation of cytoskeletal dynamics and upregulation of MT1-MMP
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SYSNO ASEP 0355190 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Neoplastic progression of the human breast cancer cell line G3S1 is associated with elevation of cytoskeletal dynamics and upregulation of MT1-MMP Author(s) Tolde, O. (CZ)
Rosel, D. (CZ)
Mierke, C.T. (DE)
Paňková, D. (CZ)
Folk, P. (CZ)
Veselý, Pavel (UMG-J)
Brabek, J. (CZ)Source Title International Journal of Oncology. - : Spandidos Publications - ISSN 1019-6439
Roč. 36, č. 4 (2010), s. 833-839Number of pages 7 s. Language eng - English Country GR - Greece Keywords invasiveness ; neoplastic progression ; cytoskeletal dynamics Subject RIV EB - Genetics ; Molecular Biology R&D Projects LC06061 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000275794300011 DOI 10.3892/ijo_00000560 Annotation The newly established breast cancer cell line G3S1, derived from EM-G3 breast cancer progenitors. was analyzed for functional changes related to neoplastic progression manifested by elevated invasiveness and enhanced capability to degrade gelatin. Degradation of gelatin and invasiveness of G3S1 cells was found to be dependent on the activity of matrix proteinases and actin cytoskeletal dynamics. Therefore, the expression and activity of these proteases was compared in G3S1 and EM-G3 cells. Despite enhanced capability of G3S1 cells to degrade gelatin. these cells exhibited lower levels of secreted extracellular matrix degrading proteases than parental EM-G3 cells. However, the expression of membrane-bound MT1-MMP was strongly elevated in G3S1 cells. While the degradation of gelatin was associated with invadopodia-like structures in both EM-G3 and G3S1 cells, the cytoskeletal remodeling dynamics was greatly elevated in G3S1 cells. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
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