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Restoration of spermatogenesis after transplantation of c-Kit positive testicular cells in the fowl
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SYSNO ASEP 0355186 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Restoration of spermatogenesis after transplantation of c-Kit positive testicular cells in the fowl Author(s) Trefil, P. (CZ)
Bakst, M.R. (US)
Yan, H. (CN)
Hejnar, Jiří (UMG-J) RID
Kalina, J. (CZ)
Mucksová, J. (CZ)Source Title Theriogenelogy. - : Elsevier - ISSN 0093-691X
Roč. 74, č. 9 (2010), s. 1670-1676Number of pages 7 s. Language eng - English Country US - United States Keywords chicken ; transplantation ; c-Kit receptor Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA523/07/1171 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000284499600019 DOI 10.1016/j.theriogenology.2010.07.002 Annotation Transplantation of male germ line cells Into sterilized recipients has been used in mammals for conventional breeding as well as for transgenesis We have previously adapted this approach for the domestic chicken and we present now an improvement of the germ cell transplantation technique by using an enriched subpopulation of c-Kit positive spermatogonia as donor cells Dispersed c-Kit positive testicular cells from 16 to 17 week-old pubertal donors were transplanted by injection directly into the testes of recipient males sterilized by repeated gamma irradiation We describe the repopulation of the recipient s testes with c Kit positive donor testicular cells, which resulted in the production of functional heterologous spermatozoa Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
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