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Fluorescent in situ hybridization of mitochondrial DNA and RNA

SYSNO ASEP	0355103
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Fluorescent in situ hybridization of mitochondrial DNA and RNA
Author(s)	Alán, Lukáš (FGU-C)_{RID, ORCID} Zelenka, Jaroslav (FGU-C)_RID Ježek, Jan (FGU-C)_{RID, ORCID} Dlasková, Andrea (FGU-C)_{RID, ORCID} Ježek, Petr (FGU-C)_{RID, ORCID}
Source Title	Acta Biochimica Polonica - ISSN 0001-527X Roč. 57, č. 4 (2010), s. 403-408
Number of pages	6 s.
Language	eng - English
Country	PL - Poland
Keywords	mitochondrial DNA and RNA ; nucleoids of mitochondrial DNA ; molecular beacon fluorescent hybridization probes
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GAP302/10/0346 GA ČR - Czech Science Foundation (CSF)
	GPP304/10/P204 GA ČR - Czech Science Foundation (CSF)
	KJB500110902 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50110509 - FGU-C (2005-2011)
UT WOS	000285726300004
Annotation	To reveal nucleic acid localization in mitochondria, we designed molecular beacon fluorescent probes against: i) the light strand complementary to ND5 mitochondrial DNA (mtDNA) gene (annealing also to corresponding mRNA); ii) displacement (D) loop 7S DNA (annealing also to parallel heavy strand mtDNA and corresponding light strand transcript); iii) the proximal D-loop heavy strand displaced by the light strand promoter minor RNA. Confocal microscopy demonstrated ND5 probe spreading (less for other probes) in mitochondrial reticulum tubules but upon RNase A treatment all probes contoured mtDNA nucleoid localization. DNase I spread the signal over mitochondrial tubules
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2011

Number of the records: 1