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Catalytic activity of matrix metalloproteinase-19 is essential for tumor suppressor and anti-angiogenic activities in nasopharyngeal carcinoma
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SYSNO ASEP 0354940 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Catalytic activity of matrix metalloproteinase-19 is essential for tumor suppressor and anti-angiogenic activities in nasopharyngeal carcinoma Author(s) Chan, K.C. (CN)
Ko, J.M. (CN)
Lung, H.L. (CN)
Sedláček, Radislav (UMG-J) RID
Zhang, Z.F. (CN)
Luo, D.Z. (CN)
Feng, Z.B. (CN)
Chen, S. (CN)
Chen, H. (CN)
Chan, K.W. (CN)
Tsao, S.W. (CN)
Chua, D.T. (CN)
Zabarovsky, E.R. (SE)
Stanbridge, E.J. (US)
Lung, M.L. (CN)Source Title International Journal of Cancer. - : Wiley - ISSN 0020-7136
Roč. 129, č. 8 (2011), s. 1826-1837Number of pages 12 s. Language eng - English Country DE - Germany Keywords MMP19 ; nasopharyngeal carcinoma ; tumor suppressor gene ; angiogenesis Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000294224300004 DOI 10.1002/ijc.25855 Annotation The association of matrix metalloproteinase-19 (MMP19) in the development of nasopharyngeal carcinoma (NPC) was identified from differential gene profiling, which showed MMP19 was one of the candidate genes down-regulated in the NPC cell lines. In this study, analysis showed MMP19 was down-regulated in all seven NPC cell lines and in 69.7% of primary NPC specimens. Allelic deletion and promoter hypermethylation contribute to MMP19 down-regulation. In the in vivo tumorigenicity assay, only the wild-type, but not mutant, MMP19 transfectants suppress tumor formation in nude mice. In the in vitro colony formation assay, WT MMP19 dramatically reduces colony-forming ability of NPC cell lines. In the tube formation assay secreted WT MMP19, but not mutant MMP19, induces reduction of tube-forming ability in endothelial cells with decreased vascular endothelial growth factor. The anti-angiogenic activity of WT MMP19 is correlated with suppression of tumor formation. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2012
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