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Stress Proteins in the Cytoplasmic Membrane Fraction of Bacillus subtilis
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SYSNO ASEP 0354589 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Stress Proteins in the Cytoplasmic Membrane Fraction of Bacillus subtilis Author(s) Petráčková, Denisa (MBU-M) RID, ORCID
Šemberová, L. (CZ)
Halada, Petr (MBU-M) RID, ORCID
Svoboda, Petr (FGU-C) RID, ORCID
Svobodová, J. (CZ)Source Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 55, č. 5 (2010), s. 427-434Number of pages 8 s. Language eng - English Country CZ - Czech Republic Keywords FUNCTION SIGMA-FACTOR ; SACCHAROMYCES-CEREVISIAE ; IDENTIFICATION Subject RIV EE - Microbiology, Virology R&D Projects LC07017 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) AV0Z50110509 - FGU-C (2005-2011) UT WOS 000282873200004 DOI 10.1007/s12223-010-0072-z Annotation Stress proteomes of the cytoplasmic membrane fraction of Bacillus subtilis trp (C2)-exposed to acid pH and ethanol were characterized. Although these stress factors impair the cell function in a specific manner, they share the ability to denature proteins. Therefore, specific and general stress proteins in the membranes were investigated. Both ethanol (3 percent) and pH 5.0 increase the doubling time from 17 to 25 min. Isolated cytoplasmic membranes were subjected to an optimized 2D PAGE analysis which permitted the separation and analysis of 450 distinct protein spots. Two alternative methods of protein detection were compared, i.e. silver staining and (35)S-L-methionine pulse labeling; the stress induced proteins were identified by MALDI-TOF MS. After ethanol stress, five proteins were increased, viz. YdaP, Ctc, YfhM, YjcH and YwaC. Acid stress proteins were AcoB, YkwC, SodA, YjcH and YwaC. Proteins YjcH and YwaC were increased after ethanol as well as acid pH treatment Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
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