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Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications for Taxonomy and Ecophysiology

SYSNO ASEP	0354405
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications for Taxonomy and Ecophysiology
Author(s)	Burdíková, Zuzana (FGU-C)_RID Čapek, Martin (FGU-C)_{RID, ORCID} Ostašov, Pavel (FGU-C) Machač, Jiří (BU-J) Pelc, Radek (FGU-C)_RID Mitchell, E.A.D. (CH) Kubínová, Lucie (FGU-C)_{RID, ORCID}
Source Title	Microscopy and Microanalysis. - : Cambridge University Press - ISSN 1431-9276 Roč. 16, č. 6 (2010), s. 735-746
Number of pages	12 s.
Language	eng - English
Country	US - United States
Keywords	testate amoebae ; confocal microscopy ; environmental scanning electron microscopy
Subject RIV	JD - Computer Applications, Robotics
R&D Projects	LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA102/08/0691 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50110509 - FGU-C (2005-2011)
	AV0Z60050516 - BU-J (2005-2011)
UT WOS	000284715300010
DOI	10.1017/S1431927610094031
Annotation	In our study we applied both traditional and advanced microscopic imaging techniques to study testate amoebae (TA) shell and cell morphology. When compared with scanning electron microscopy (SEM) or environmental SEM (ESEM), confocal laser scanning microscopy (CLSM) and two-photon excitation microscopy (TPEM) offered several additional advantages for investigations requiring information of morphological characteristics, useful for taxonomical and ecophysiological research: (1) CLSM and TPEM allow imaging of inner structures of TA in contrast to SEM or ESEM. (2) CLSM and TPEM can simplify and refine studies of morphology. (3) CLSM and TPEM allow us to obtain data that can be used for stereological estimations of volumes of TA. (4) Fluorescence markers allow us to see structures not visible in transmitting light
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2011

Number of the records: 1