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Separation of PCBs by liquid chromatography on reversed phase sub-2-micron particle columns

SYSNO ASEP	0354246
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Separation of PCBs by liquid chromatography on reversed phase sub-2-micron particle columns
Author(s)	Olšovská, Jana (MBU-M) Křesinová, Zdena (MBU-M)_RID Flieger, Miroslav (MBU-M)_ORCID Cajthaml, Tomáš (MBU-M)_{RID, ORCID}
Source Title	Talanta. - : Elsevier - ISSN 0039-9140 Roč. 80, č. 5 (2010), s. 1849-1855
Number of pages	7 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Ultra high-performance liquid chromatography ; PCB ; Congener analysis
Subject RIV	EE - Microbiology, Virology
R&D Projects	2B06156 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA525/09/1058 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000275612500041
DOI	10.1016/j.talanta.2009.10.033
Annotation	A new ultra high-performance liquid chromatography method with UV detection was examined for detection and separation of polychlorinated biphenyls. This included optimization of separation conditions for two model mixtures containing seven and fifteen most relevant congeners, comparison of three types of reversed phase sub-2-micron particle sized columns and assessment of system suitability under the optimized conditions. Calibration curves determined in the range from 0.5 to 50.0_g/mL exhibited correlation coefficients ranging from 0.997 to 0.999. Lower limits of detection ranged from 0.1 to 0.5 ppm. The most efficient Grace C18 column filled with 1.5_m particles was then tested to separate the complex commercial mixture Delor 103, where the elution order was confirmed by GC–MS. 13 individual congeners were separated and some of the other co-eluting congeners could be resolved using another separation dimension performed with a mass spectrometry detector
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2011

Number of the records: 1