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Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae

SYSNO ASEP	0354019
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae
Author(s)	Burdíková, Zuzana (FGU-C)_RID Čapek, Martin (FGU-C)_{RID, ORCID} Ostašov, Pavel (FGU-C) Mitchell, E.A.D. (CH) Machač, Jiří (BU-J) Kubínová, Lucie (FGU-C)_{RID, ORCID}
Source Title	Microscopy and Microanalysis. - : Cambridge University Press - ISSN 1431-9276 Roč. 16, Suppl.2 (2010), s. 1142-1143
Number of pages	2 s.
Action	Microscopy and Microanalysis 2010
Event date	01.08.2010-05.08.2010
VEvent location	Portland
Country	US - United States
Event type	WRD
Language	eng - English
Country	US - United States
Keywords	testate amoebae ; confocal microscopy ; two-photon microscopy
Subject RIV	BH - Optics, Masers, Lasers
R&D Projects	LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA102/08/0691 GA ČR - Czech Science Foundation (CSF)
	GA304/09/0733 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50110509 - FGU-C (2005-2011)
	AV0Z60050516 - BU-J (2005-2011)
DOI	10.1017/S1431927610060897
Annotation	We applied CLSM and Two-Photon Excitation (TPE) microscopy techniques to visualize testate amoebae (TA), their test, morphology and physiology. TA are usually examined using SEM and E-SEM. It is not possible to acquire images of living TA and physiology of the amoeba inside the test by SEM or E-SEM. The goal of the study was to examine the potential of CLSM and TPE for imaging of TA. We tried 17 fluorescent dyes to label different structures of TA. CLSM enabled us to acquire images of TA from depths up to 40 μm, whereas TPE was able to penetrate to 60 μm. Stereological methods were employed to estimate the volume of the biomass of TA visualized by CLSM
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2011

Number of the records: 1