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Biochemical and binding characteristics of boar epididymal fluid proteins
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SYSNO ASEP 0353966 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biochemical and binding characteristics of boar epididymal fluid proteins Author(s) Maňásková-Postlerová, Pavla (BTO-N) ORCID
Davidová, Nina (BTO-N)
Jonáková, Věra (BTO-N) RIDNumber of authors 3 Source Title Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier - ISSN 1570-0232
Roč. 879, č. 1 (2011), s. 100-106Number of pages 7 s. Language eng - English Country NL - Netherlands Keywords epididymal proteins ; RP HPLC ; biotin-labeled ligands Subject RIV CE - Biochemistry R&D Projects GA303/09/1285 GA ČR - Czech Science Foundation (CSF) 1M06011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GD523/08/H064 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000286539900015 DOI 10.1016/j.jchromb.2010.11.023 Annotation Proteins and glycoproteins of boar epididymal fluid may be adsorbed on the sperm surface and participate in the sperm maturation, in sperm capacitation, gamete recognition, binding and fusion. Boar epididymal fluid proteins were separated by size exclusion chromatography and by RP-HPLC. The fractions were characterized by SDS-PAGE and proteins were transfered to nitrocellulose membranes and tested for the interaction with biotin-labeled ligands: glycoproteins of zona pellucida (ZP), hyaluronic acid and heparin. Changes in the interaction of epididymal sperm were studied by ELBA. The affinity of epididymal proteins (12-17 kDa, 23 kDa) to heparin and hyaluronic acid suggests their binding ability to oviductal proteoglycans. Epididymal proteins of 12-18 kDa interacted with ZP glycoproteins. We identified Crisp3-like protein. We showed the ability of epididymal fluid fractions to change the interaction of the epididymal sperm surface with ZP glycoproteins, hyaluronic acid and heparin. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2012
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