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Multiplex PCR for Differential Identification of Broad Tapeworms (Cestoda: Diphyllobothrium) Infecting Humans

SYSNO ASEP	0353453
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Multiplex PCR for Differential Identification of Broad Tapeworms (Cestoda: Diphyllobothrium) Infecting Humans
Author(s)	Wicht, B. (CH) Yanagida, T. (JP) Scholz, Tomáš (BC-A)_{RID, ORCID} Ito, A. (JP) Jiménez, J. A. (PE) Brabec, Jan (BC-A)_{RID, ORCID}
Source Title	Journal of Clinical Microbiology - ISSN 0095-1137 Roč. 48, č. 9 (2010), s. 3111-3116
Number of pages	6 s.
Language	eng - English
Country	US - United States
Keywords	MOLECULAR EVIDENCE ; PACIFIC SALMON ; NIHONKAIENSE ; Diphyllobothrium ; multiplex PCR ; the cytochrome c oxidase subunit 1 ; mitochondrial DNA
Subject RIV	GJ - Animal Vermins ; Diseases, Veterinary Medicine
R&D Projects	LC522 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000281480400010
DOI	10.1128/JCM.00445-10
Annotation	The specific identification of broad tapeworms (genus Diphyllobothrium) infecting humans is very difficult to perform by morphological observation. Molecular analysis by PCR and sequencing represents the only reliable tool to date to identify these parasites to the species level. Due to the recent spread of human diphyllobothriosis in several countries, a correct diagnosis has become crucial to better understand the distribution and the life cycle of human-infecting species as well as to prevent the introduction of parasites to disease-free water systems. Nevertheless, PCR and sequencing are too complicated, long, and expensive for routine diagnostics. In the present study we optimized a cheap and rapid molecular test for the differential identification of the most common Diphyllobothrium species infecting humans (D. latum, D. dendriticum, D. nihonkaiense, and D. pacificum), based on a multiplex PCR with the cytochrome c oxidase subunit 1 gene of mitochondrial DNA.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1