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The transmembrane adaptor protein NTAL signals to mast cell cytoskeleton via the small GTPase Rho
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SYSNO ASEP 0353421 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The transmembrane adaptor protein NTAL signals to mast cell cytoskeleton via the small GTPase Rho Author(s) Tůmová, Magda (UMG-J)
Koffer, Anna (UMG-J)
Šimíček, Michal (UMG-J)
Dráberová, Lubica (UMG-J) RID
Dráber, Petr (UMG-J) RIDSource Title European Journal of Immunology. - : Wiley - ISSN 0014-2980
Roč. 40, č. 11 (2010), s. 3235-3245Number of pages 11 s. Language eng - English Country DE - Germany Keywords cell activation ; cytoskeleton ; mast cells Subject RIV EB - Genetics ; Molecular Biology R&D Projects 1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GD204/05/H023 GA ČR - Czech Science Foundation (CSF) GA301/09/1826 GA ČR - Czech Science Foundation (CSF) GAP302/10/1759 GA ČR - Czech Science Foundation (CSF) KAN200520701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000284059000028 DOI 10.1002/eji.201040403 Annotation The transmembrane adaptor protein NTAL participates in signalosome assembly in hematopoietic cells, but its exact role in cell physiology remains enigmatic. This study shows that mast cells from NTAL-deficient mice, responding to Ag alone or in combination with SCF, exhibit reduced spreading on fibronectin, enhanced filamentous actin depolymerization and enhanced migration towards Ag relative to WT cells. No such differences between WT and NTAL(-/-) cells were observed when SCF alone was used as activator. Next the activities of two small GTPases, Rac and Rho, which are important regulators of actin polymerization were examined. Stimulation with Ag and/or SCF enhanced activity of Rac (1,2,3) in both NTAL(-/-) and WT cells. In contrast, RhoA activity decreased and this trend was much faster and more extensive in NTAL(-/-) cells, indicating a positive regulatory role of NTAL in the recovery of RhoA activity. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
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