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Attenuation of vaccinia virus by the expression of human Flt3 ligand
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SYSNO ASEP 0353377 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Attenuation of vaccinia virus by the expression of human Flt3 ligand Author(s) Žurková, K. (CZ)
Hainz, P. (CZ)
Kryštofová, J. (CZ)
Kutinová, L. (CZ)
Šanda, Miloslav (UOCHB-X)
Němečková, Š. (CZ)Number of authors 6 Source Title Virology Journal
Roč. 7, č. 1 (2010), 109/1-109/15Number of pages 15 s. Language eng - English Country GB - United Kingdom Keywords vaccinia virus ; antibodies ; virulence Subject RIV CE - Biochemistry CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000279552600004 DOI 10.1186/1743-422X-7-109 Annotation The expression of Flt3 ligand (FL) by recombinant vaccinia virus (rVACV) highly influenced properties of the virus in dependence on the level of expression. High production of FL driven by the strong synthetic promoter decreased the growth of rVACV in macrophage cell line J774.G8 in vitro as well as its multiplication in vivo when inoculated in mice. The inhibition of replication in vivo was mirrored in low levels of antibodies against vaccinia virus (anti-VACV) which nearly approached to the negative serum level in non-infected mice. Strong FL expression changed not only the host range of the recombinant but also the basic protein contents of virions. The major proteins - H3L and D8L - which are responsible for the virus binding to the cells, and 28 K protein that serves as a virulence factor, were changed in the membrane portion of P13-E/L-FL viral particles. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2011
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