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Bordetella Adenylate Cyclase Toxin Mobilizes Its beta(2) Integrin Receptor into Lipid Rafts to Accomplish Translocation across Target Cell Membrane in Two Steps
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SYSNO ASEP 0352882 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Bordetella Adenylate Cyclase Toxin Mobilizes Its beta(2) Integrin Receptor into Lipid Rafts to Accomplish Translocation across Target Cell Membrane in Two Steps Author(s) Bumba, Ladislav (MBU-M) RID, ORCID
Mašín, Jiří (MBU-M) RID, ORCID
Fišer, R. (CZ)
Šebo, Peter (MBU-M) RID, ORCIDSource Title PLoS Pathogens. - : Public Library of Science - ISSN 1553-7366
Roč. 6, č. 5 (2010), s. 1-15Number of pages 15 s. Language eng - English Country US - United States Keywords SHEEP ERYTHROCYTES ; BACILLUS-ANTHRACIS ; ESCHERICHIA-COLI Subject RIV EE - Microbiology, Virology R&D Projects GP310/07/P115 GA ČR - Czech Science Foundation (CSF) 1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) IAA500200914 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) 2B06161 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA310/08/0447 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50200510 - MBU-M (2005-2011) AV0Z50520701 - BTO-N (2007-2013) UT WOS 000278759900020 DOI 10.1371/journal.ppat.1000901 Annotation The adenylate cyclase toxin (CyaA) of pathogenic Bordetellae eliminates the first line of host innate immune defense. It penetrates myeloid phagocytes, such as neutrophils, macrophage or dendritic cells, and subverts their signaling by catalyzing an extremely rapid conversion of intracellular ATP to the key signaling molecule cAMP. This efficiently inhibits the oxidative burst and complement- mediated opsonophagocytic killing of bacteria, thus enabling the pathogen to colonize host airways. We show that translocation of CyaA into phagocyte cytosol occurs in two steps. The toxin first binds the integrin CD11b/CD18 and inserts into phagocyte membrane to mediate influx of calcium ions into cells. This promotes relocation of the toxin-receptor complex into specific lipid microdomains within cell membrane called rafts Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
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