Number of the records: 1
Distribution and development of the postnatal murine V delta 1 T-cell receptor repertoire
- 1.
SYSNO ASEP 0352860 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Distribution and development of the postnatal murine V delta 1 T-cell receptor repertoire Author(s) Holtmeier, W. (DE)
Gille, J. (DE)
Zeuzem, S. (DE)
Šinkora, Marek (MBU-M) RID, ORCIDSource Title Immunology - ISSN 0019-2805
Roč. 131, č. 2 (2010), s. 192-201Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords gene rearrangement ; repertoire development ; rodent Subject RIV EE - Microbiology, Virology R&D Projects GA524/07/0087 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000281554600005 DOI 10.1111/j.1365-2567.2010.03290.x Annotation Murine gamma/delta T cells express canonical V gamma 5V delta 1 chains in the epidermis and V gamma 6V delta 1 chains at reproductive sites. Both subsets carry an identical V delta 1-D delta 2-J delta 2 chain which completely lacks junctional diversity. These cells are thought to monitor tissue integrity via recognition of stress-induced self antigens. In this study, we showed by reverse transcription-polymerase chain reaction (RT-PCR), complementarity determining region 3 (CDR3) spectratyping and sequencing of the junctional regions of V delta 1 chains from C57BL/6 mice (aged 1 day to 14 months) that the canonical V delta 1-D delta 2-J delta 2 chain is also consistently present at other sites such as the thymus, gut, lung, liver, spleen and peripheral blood Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
Number of the records: 1