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Enhancement of autofluorescence of the brown-rot fungus Piptoporus betulinus by metal ions
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SYSNO ASEP 0352792 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Enhancement of autofluorescence of the brown-rot fungus Piptoporus betulinus by metal ions Author(s) Žižka, Zdeněk (MBU-M) RID, ORCID
Větrovský, Tomáš (MBU-M) ORCID, RID
Gabriel, Jiří (MBU-M) RIDSource Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 55, č. 6 (2010), s. 625-628Number of pages 4 s. Language eng - English Country CZ - Czech Republic Keywords Piptoporus betulinus ; autofluorescenc ; metal ions Subject RIV EE - Microbiology, Virology R&D Projects GA526/07/0620 GA ČR - Czech Science Foundation (CSF) LC06066 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000286466300014 DOI 10.1007/s12223-010-0101-y Annotation The autofluorescence (primary fluorescence, AF) of agar cultures of the brown-rot fungus Piptoporus betulinus was investigated in Zeiss Jenalumar and Nikon Eclipse 80i fluorescence microscopes at various excitations. The stronges AF of hyphae was found in minimal medium with glucose, where the hyphae exhibited green AF at violet (450 nm) excitation and red AF at green (570 nm) excitation. Addition of metals to cultivation media led to enhanced white-blue AF in the presence of Co (at 450 nm) and yellow to yellow-brown AF at 510 nm. When cultivated with Mn and Zn, enhanced AF of intracellular content was observed. Only a weak signal was found in the presence of Cu and Fe Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
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