Number of the records: 1

Raman spectroscopy for the characterization of algal cells

SYSNO ASEP	0352652
Document Type	A - Abstract
R&D Document Type	The record was not marked in the RIV
R&D Document Type	Není vybrán druh dokumentu
Title	Raman spectroscopy for the characterization of algal cells
Author(s)	Samek, Ota (UPT-D)_{RID, ORCID, SAI} Jonáš, Alexandr (UPT-D)_{RID, SAI, ORCID} Pilát, Zdeněk (UPT-D)_{RID, SAI, ORCID} Zemánek, Pavel (UPT-D)_{RID, SAI, ORCID} Nedbal, Ladislav (UEK-B) Trtílek, M. (CZ)
Number of authors	8
Source Title	17th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics. - Žilina : Žilinská univerzita, 2010 - ISBN 978-80-554-0238-3 S. 97
Number of pages	1 s.
Action	Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics /17./
Event date	06.09.2010
VEvent location	Liptovsky Jan
Country	SK - Slovakia
Event type	WRD
Language	eng - English
Country	SK - Slovakia
Keywords	Raman spectroscopy ; algal cells ; lipids ; iodine value
Subject RIV	BH - Optics, Masers, Lasers
R&D Projects	ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	OC08034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z20650511 - UPT-D (2005-2011)
	AV0Z60870520 - UEK-B (2005-2011)
Annotation	Raman spectroscopy can elucidate fundamental questions about intercellular variability and what governs it. Moreover, knowing the metabolic response on single cell level this can significantly contribute to the study and use of microalgae in systems biology and biofuel technology. Raman spectroscopy is capable to measure nutrient dynamics and metabolism in vivo, in real-time, label free making it possible to monitor/evaluate population variability. Also, degree of unsaturation of the algae oil (iodine value) can be measured using Raman spectra obtained from single microalgae. The iodine value is the determination of the amount of unsaturation contained in fatty acids (in the form of double bonds). Here we demonstrate the capacity of the spatially resolved Raman microspectroscopy to determine the effective iodine value in lipid storage bodies of individual living algal cells.
Workplace	Institute of Scientific Instruments
Contact	Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
Year of Publishing	2011

Number of the records: 1