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Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth
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SYSNO ASEP 0352642 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth Author(s) Kameník, Zdeněk (MBU-M) RID, ORCID
Hadacek, F. (AT)
Marečková, M. (CZ)
Ulanová, Dana (MBU-M)
Kopecký, J. (CZ)
Chobot, V. (AT)
Plháčková, Kamila (MBU-M) RID
Olšovská, Jana (MBU-M)Source Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1217, č. 51 (2010), s. 8016-8025Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords UHPLC ; high-throughput fingerprinting ; secondary metabolites Subject RIV CB - Analytical Chemistry, Separation R&D Projects 2B08064 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000285429700012 DOI 10.1016/j.chroma.2010.08.031 Annotation A fingerprinting method for chemical screening of microbial metabolites, potential antibiotics, in spent cultivation broths is described. The method is based on high-throughput ultra-high-performance liquid chromatography (UHPLC) separation with UV detection (photodiode array detector). Thirteen antibiotic standards and four cultivation broths were used for the method development. The comparison of ten liquid–liquid and solid phase extraction protocols for sample clean-up and pre-concentration revealed that Oasis HLB C18 sorbent gives the best recoveries. The Acquity BEH C18 chromatographic column was chosen for the samples separation with respect to its universality, selectivity, efficiency and robustness. The method is presented by two 3D fingerprints for every sample that was obtained under different, acidic and alkaline, UHPLC conditions Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
Number of the records: 1