Number of the records: 1

Biotransformations with nitrilases

SYSNO ASEP	0352304
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Biotransformations with nitrilases
Author(s)	Martínková, Ludmila (MBU-M)_{RID, ORCID} Křen, Vladimír (MBU-M)_{RID, ORCID}
Source Title	Current Opinion in Chemical Biology. - : Elsevier - ISSN 1367-5931 Roč. 14, č. 2 (2010), s. 130-137
Number of pages	8 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	ENZYME-PROMOTED DESYMMETRISATION ; PSEUDOMONAS-FLUORESCENS EBC191 ; SUBSTRATE-SPECIFICITY
Subject RIV	CE - Biochemistry
R&D Projects	IAA500200708 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	LC06010 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	OC09046 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	FT-TA5/043 GA MPO - Ministry of Industry and Trade (MPO)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000277110800004
DOI	10.1016/j.cbpa.2009.11.018
Annotation	The range of possible nitrilase applications has been recently broadened but in most cases the parameters of the reactions need to be improved to establish viable industrial processes. To achieve this goal, several methods have been used, primarily in screening for enzymes from new sources, enzyme improvement, substrate structure modification, medium engineering, and variation in process parameters. New nitrilases have been obtained by for example genome mining and the metagenomic approach. Protein engineering revealed targets for altering substrate specificity, activity or enantioselectivity, and for changing the acid:amide ratio in the nitrilase product
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2011

Number of the records: 1