Number of the records: 1  

Raman spectroscopy for the characterization of algal cells

  1. 1.
    SYSNO ASEP0352210
    Document TypeC - Proceedings Paper (int. conf.)
    R&D Document TypeConference Paper
    TitleRaman spectroscopy for the characterization of algal cells
    Author(s) Samek, Ota (UPT-D) RID
    Jonáš, Alexandr (UPT-D) RID
    Pilát, Zdeněk (UPT-D) RID
    Zemánek, Pavel (UPT-D) RID
    Nedbal, Ladislav (UEK-B)
    Tříska, Jan (UEK-B) RID
    Kotas, Petr (UEK-B) RID
    Trtílek, M. (CZ)
    Number of authors8
    Source Title17th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE Vol. 7746). - Bellingham : SPIE, 2010 - ISBN 978-0-8194-8236-5
    Pages77460x: 1-7
    Number of pages7 s.
    ActionSlovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics /17./
    Event date06.09.2010
    VEvent locationLiptovsky Jan
    CountrySK - Slovakia
    Event typeWRD
    Languageeng - English
    CountryUS - United States
    KeywordsRaman spectroscopy ; algal cells ; lipids ; iodine value
    Subject RIVBH - Optics, Masers, Lasers
    R&D ProjectsED0017/01/01 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    OC08034 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    CEZAV0Z20650511 - UPT-D (2005-2011)
    AV0Z60870520 - UEK-B (2005-2011)
    UT WOS000291389400033
    AnnotationRaman spectroscopy can elucidate fundamental questions about intercellular variability and what governs it. Moreover, knowing the metabolic response on single cell level this can significantly contribute to the study and use of microalgae in systems biology and biofuel technology. Raman spectroscopy is capable to measure nutrient dynamics and metabolism in vivo, in real-time, label free making it possible to monitor/evaluate population variability. Also, degree of unsaturation of the algae oil (iodine value) can be measured using Raman spectra obtained from single microalgae. The iodine value is the determination of the amount of unsaturation contained in fatty acids (in the form of double bonds). Here we demonstrate the capacity of the spatially resolved Raman microspectroscopy to determine the effective iodine value in lipid storage bodies of individual living algal cells.
    WorkplaceInstitute of Scientific Instruments
    ContactJan Slaměník, jan@isibrno.cz, Tel.: 541 514 205 ; Miluše Langová, langova@isibrno.cz
    Year of Publishing2011