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Development of a fast LC-MS/MS method for quantification of rilmenidine in human serum: elucidation of fragmentation pathways by HRMS
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SYSNO ASEP 0352155 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Development of a fast LC-MS/MS method for quantification of rilmenidine in human serum: elucidation of fragmentation pathways by HRMS Author(s) Chytil, L. (CZ)
Cvačka, Josef (UOCHB-X) RID, ORCID
Marešová, V. (CZ)
Štrauch, B. (CZ)
Widimský jr., J. (CZ)
Štícha, M. (CZ)
Slanař, O. (CZ)Number of authors 7 Source Title Journal of Mass Spectrometry. - : Wiley - ISSN 1076-5174
Roč. 45, č. 10 (2010), s. 1179-1185Number of pages 7 s. Language eng - English Country GB - United Kingdom Keywords serum ; quantification ; HRMS Subject RIV CC - Organic Chemistry CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000283610200011 DOI 10.1002/jms.1809 Annotation Rilmenidine is an alpha 2 adrenoreceptor agonist used in the treatment of mild and moderate hypertension. A fast and accurate liquid chromatographic method with tandem mass spectrometric detection has been validated in order to assure quantification of rilmenidine in human serum. The fragmentation pathway of protonated rilmenidine was studied using high-resolution mass spectrometry (HRMS). This study compared selectivity, linearity, accuracy, precision, extraction efficiency, matrix effect and sensitivity using common liquid–liquid extraction (LLE) and solid-phase extraction (SPE) procedures. Several differences between the LLE and SPE have been observed in terms of linearity, accuracy, precision and matrix effect. The developed method involving SPE was found to be accurate, reproducible, robust and suitable for quantitative analysis of rilmenidine in serum samples obtained from patients under antihypertensive treatment. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2011
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