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Conserved mechanism of Wnt signaling function in the specification of vulval precursor fates in C. elegans and C. briggsae

SYSNO ASEP	0352047
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Conserved mechanism of Wnt signaling function in the specification of vulval precursor fates in C. elegans and C. briggsae
Author(s)	Seetharaman, A. (CA) Cumbo, P. (CA) Bojanala, Nagagireesh (BC-A) Gupta, B. P. (CA)
Source Title	Developmental Biology. - : Elsevier - ISSN 0012-1606 Roč. 346, č. 1 (2010), s. 128-139
Number of pages	12 s.
Language	eng - English
Country	US - United States
Keywords	Nematode ; C. elegans ; C. briggsae ; Vulval development ; Signal transduction ; Wnt signaling
Subject RIV	EB - Genetics ; Molecular Biology
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000281930900011
DOI	10.1016/j.ydbio.2010.07.003
Annotation	To understand the function and evolution of Wnt signaling in Caenorhabditis nematodes we focused on C. briggsae, a species that is substantially divergent from C. elegans in terms of the evolutionary time scale yet shares almost identical morphology. We isolated mutants in C briggsae that display multiple pseudo-vulvae resulting from ectopic VPC induction. We cloned one of these loci and found that it encodes an Axin homolog, Cbr-PRY-1. Our genetic studies revealed that Cbr-pry-1 functions upstream of the canonical Wnt pathway components Cbr-bar-1 (beta-catenin) and Cbr-pop-1(tcf/lef) as well as the Hox target Cbr-lin-39 (Dfd/Scr). We further characterized the pry-1 vulval phenotype in C briggsae and C elegans using 8 cell fate markers, cell ablation, and genetic interaction approaches. Our results show that ectopically induced VPCs in pry-1 mutants adopt 2 degrees fates independently of the gonad-derived inductive and LIN-12/Notch-mediated lateral signaling pathways.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1