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Immobilization of CGTases from Bacillus circulans and Bacillus pseudalcaliphilus on aminosilane and PEI modified magnetic nanoparticles and modified silica particles
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SYSNO ASEP 0351749 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Immobilization of CGTases from Bacillus circulans and Bacillus pseudalcaliphilus on aminosilane and PEI modified magnetic nanoparticles and modified silica particles Author(s) Ivanova, V. (BG)
Tonkova, A. (BG)
Atanassova, N. (BG)
Šafaříková, Miroslava (UEK-B) RID, SAI
Hristov, J. (BG)Number of authors 5 Source Title International Review of Chemical Engineering, Special Selection on 1st Conference on Chemical Engineering and Advanced Materials - ISSN 2035-1755
Roč. 2, č. 2 (2010), s. 278-288Number of pages 11 s. Action 1st Conference on Chemical Engineering and Advanced Materials Event date 23.11.2009-08.12.2009 VEvent location Naples Country IT - Italy Event type EUR Language eng - English Country BG - Bulgaria Keywords cyclodextrin glucanotransferase ; immobilization ; magnetic particles Subject RIV CE - Biochemistry R&D Projects 2A-1TP1/094 GA MPO - Ministry of Industry and Trade (MPO) CEZ AV0Z60870520 - UEK-B (2005-2011) Annotation The extracellular enzymes cyclodextrin glucanotransferases produces by Bacillus circulans ATCC 21783, Bacillus pseudoalcaliphilus strain 8SB and Bacillus pseudoalcaliphilus strain 20RF were immobilized by covalent linking via glutaraldehyde to silanized magnetic nanoparticles treated with PEI, silanized magnetite and magnetic nanoprarticles, and commercionally functionalized silica nano- and microparticles in order to study the immobilization capacities of the carriers, the activity recovery and some biochemical characteristics of the enzymes after immobilization. The immobilized enzymes showed a high operational stability after twelve re-uses. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2011
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