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Regulation of the Chlamydomonas Cell Cycle by a Stable, Chromatin-Associated Retinoblastoma Tumor Suppressor Complex
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SYSNO ASEP 0351683 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Regulation of the Chlamydomonas Cell Cycle by a Stable, Chromatin-Associated Retinoblastoma Tumor Suppressor Complex Author(s) Olson, B. J. S. C. (US)
Oberholzer, M. (US)
Li, Y. (US)
Zones, J. M. (US)
Kohli, H. S. (US)
Bišová, Kateřina (MBU-M) RID
Fang, S. - Ch. (US)
Meisenhelder, J. (US)
Hunter, T. (US)
Umen, J. G. (US)Source Title Plant Cell. - : Oxford University Press - ISSN 1040-4651
Roč. 22, č. 10 (2010), s. 3331-3347Number of pages 7 s. Language eng - English Country US - United States Keywords GENOME-WIDE ANALYSIS ; TRANSCRIPTION FACTOR ; GENE-PRODUCT Subject RIV EE - Microbiology, Virology CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000284580400013 DOI 10.1105/tpc.110.076067 Annotation We examined the cell cycle dynamics of the retinoblastoma (RB) protein complex in the unicellular alga Chlamydomonas reinhardtiithat has single homologs for each subunit—RB, E2F, and DP. We found that Chlamydomonas RB (encoded by MAT3) is a cellcycle–regulated phosphoprotein, that E2F1-DP1 can bind to a consensus E2F site, and that all three proteins interact in vivo to form a complex that can be quantitatively immunopurified. Yeast two-hybrid assays revealed the formation of a ternary complex between MAT3, DP1, and E2F1 that requires a C-terminal motif in E2F1 analogous to the RB binding domain of plant and animal E2Fs Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
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