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Involvement of carotenoids in the synthesis and assembly of protein subunits of photosynthetic reaction centers of Synechocystis sp. PCC 6803
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SYSNO ASEP 0351636 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Involvement of carotenoids in the synthesis and assembly of protein subunits of photosynthetic reaction centers of Synechocystis sp. PCC 6803 Author(s) Sozer, O. (HU)
Komenda, Josef (MBU-M) RID, ORCID
Ughy, B. (HU)
Domonkos, I. (HU)
Laczkó-Dobos, H. (HU)
Malec, P. (PL)
Gombos, Z. (HU)
Kis, M. (HU)Source Title Plant and Cell Physiology. - : Oxford University Press - ISSN 0032-0781
Roč. 51, č. 5 (2010), s. 823-835Number of pages 13 s. Language eng - English Country JP - Japan Keywords Carotenoidless mutant ; crtB ; Membrane protein synthesis Subject RIV EE - Microbiology, Virology R&D Projects IAA400200801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000277734900013 DOI 10.1093/pcp/pcq031 Annotation The crtB gene of Synechocystis sp. PCC 6803, encoding phytoene synthase, was inactivated in the Delta crtH mutant to generate a carotenoidless Delta crtH/B double mutant. Delta crtH mutant cells were used because they had better transformability than wild-type cells, most probably due to their adaptation to partial carotenoid deficiency. Cells of the Delta crtH/B mutant were light sensitive and could grow only under light-activated heterotrophic growth conditions in the presence of glucose. Carotenoid deficiency did not significantly affect the cellular content of phycobiliproteins while the chlorophyll content of the mutant cells decreased Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
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