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Access to enantiopure aromatic epoxides and diols using epoxide hzdrolases derived from total biofilter DNA
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SYSNO ASEP 0351499 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Access to enantiopure aromatic epoxides and diols using epoxide hzdrolases derived from total biofilter DNA Author(s) Kotík, Michael (MBU-M) RID, ORCID
Štěpánek, Václav (MBU-M) RID, ORCID
Grulich, Michal (MBU-M) RID
Kyslík, Pavel (MBU-M) RID, ORCID
Archelas, A. (FR)Source Title Journal of Molecular Catalysis B-Enzymatic. - : Elsevier - ISSN 1381-1177
Roč. 65, 1-4 (2010), s. 41-48Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords Chiral resolution ; Enantioconvergent reaction ; Enantioselectivity Subject RIV CE - Biochemistry R&D Projects GA204/06/0458 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000278926300008 DOI 10.1016/j.molcatb.2010.01.016 Annotation Two genes encoding functional epoxide hydrolases were retrieved from total DNA isolated from biofilter-derived biomass, using PCR with epoxide hydrolase-specific degenerate primers followed by genome-walking PCR. The resulting recombinant epoxide hydrolases were expressed in Escherichia coli and characterised Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
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