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High-performance liquid chromatography–off line mass spectrometry analysis of anthraquinones produced by Geosmithia lavendula
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SYSNO ASEP 0351441 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title High-performance liquid chromatography–off line mass spectrometry analysis of anthraquinones produced by Geosmithia lavendula Author(s) Stodůlková, Eva (MBU-M) ORCID
Man, Petr (MBU-M) RID, ORCID
Kolařík, Miroslav (MBU-M) RID, ORCID
Flieger, Miroslav (MBU-M) ORCIDSource Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1217, č. 40 (2010), s. 6296-6302Number of pages 7 s. Language eng - English Country NL - Netherlands Keywords High-performance liquid chromatography ; Mass spectrometry ; Anthraquinones Subject RIV EE - Microbiology, Virology R&D Projects 2B08064 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) KAN200200651 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000282725800020 DOI 10.1016/j.chroma.2010.08.009 Annotation Lilac coloured species of Geosmithia lavendula produce a mixture of polyhydroxylated anthraquinones under condition of submerged fermentation. Three pigments had been isolated and identified earlier as a 1,3,6,8-tetrahydroxyanthraquinone (compound 7), rhodolamprometrin (1-acetyl 2,4,5,7-tetrahydroxyanthraquinone; compound 5), and 1-acetyl 2,4,5,7,8-penthahydroxyanthraquinone (compound 4). A new HPLC method was developed for the separation of three known and ten new anthraquinone pigments. In addition, five new pigments were determined by FTMS as coeluting impurities. The analyses were performed on a reversed phase column using gradient elution with a mobile phase system consisting of phosphate buffer (50mM, pH 2.0) and acetonitrile. The structure evaluation was based namely on FTMS and UV–VIS spectrometry. The developed procedure was used for the determination of individual anthraquinones in fermentation broth of G. lavendula after 14 days of cultivation Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
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